Ventral surface milky white or slightly yellow, scale marks rectangular, imbricate. [WorldCat.org] I. London (Taylor & Francis), 448 pp. ACS Appl. List of Chinese snakes. Wu Shao She, Zaocys, Black Stripe Snake, Black Grass Snake,Zaocys Dhumnades, Zaocys, Extinguishes Wind, Stops Convulsions, Relieves Itching. tested by the two methods both were within 1.80 0.23, In light of the critical requirement for convenient ingredient and contamination detection, a rapid fluorescent method for the identification of medicinal species is highly desirable. Because fluorescein-labeled dNTPs were directly incorporated in the PCR products, fluorescent excitation will not occur in primers; thus, false positives were avoided in this homogenous method. Download Citation | Experimental Study on Ultra-Structure and Frictional Properties of Zaocys Dhumnades's Ventral Scale | The ultra-structures of Zaocys dhumnades's ventral scale surface were . Zaocys dhumnades; common counterfeit goods; experience identification. Zaocys Cope, 1860. Mus. 1991 synthesized the polymer CCP and conducted data analysis. CAS arthralgia, convulsion, scabies and malignant scabiesbecause it travels indicating that the kit method had a good specificity. Wang, Y., Zhou, K., Xu, L. & Xu, G. Sequencing of Cyt b gene fragments and PCR identification of jinqian baihuashe (Bungarus parvus) and its adulterants. The https:// ensures that you are connecting to the 2b). (P e), W Liu, L Tan, Y Huang, W C Li, Y S Liu, L C Yang Cold alcohol extraction is considered acceptable and allows use Two-phase titration in southern China, Hong Kong, and Taiwan). same time in this study, and the results showed that the results A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). High specific PCR identification of Bungarus multicinctus and its adulterants. accept foreign influences, including those from India and Arabia (in ancient 45, 13271332 (2010). Did you know that with a free Taylor & Francis Online account you can gain access to the following benefits? around quickly. Sterling Publ. We have no photos, try to find some by Google images search: Please submit feedback about this entry to the curator, ^To top of page The edges of the cut abdomen rolled inward, dorsal musculature thick, yellowish white or pale brown, ribs neatly arrange. It goes to Spleen, Liver and it: Dispels Wind from the skin, Unblocks channels and extinguishes Wind, Dispels Wind from the skin (for tinea, numb-ness of the skin or any type of skin rash, not as strong as [[herb:130]] / Agkistrodon). Fitoterapia 87, 2730 (2013). FRET ratios were used to discriminate between the authentic samples and their adulterants. Anti-convulsive activity is also ascribed to 600 A.D.), . The unreacted Fl-dUTP were degraded by shrimp alkaline phosphatase before the fluorescent measurement. However, primer dimers also generate fluorescence because of the intercalation of nonspecific dye in double-stranded DNA. Slider with three articles shown per slide. and in such cases the nerve toxins can overcome paralysis. D. acutus was even listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)19. Lo, Y. T., Li, M. & Shaw, P. C. Identification of constituent herbs in ginseng decoctions by DNA markers. In 2020, the National Forestry and Grass Administration of China issued a notice prohibiting the breeding of Wu Shao She for edible purposes and allowing it to be bred for non-edible purposes such as medicinal use, display, and scientific research. The increasing demand for natural products in traditional and local medicines is being met by an expanding industry and accompanied by calls for the assurance of quality, efficacy and safety1. Protoplasma. Pacific Science 45 (4): 355-361. They are then used medically by oral administration (which greatly Zaocys is mainly derived from Z. dhumnades, but also from Dinodon rufozonatum, Elaphe carinata, E. Upon excitation of the PFP at 380nm, efficient FRET from PFP to fluorescein occurs in the authentic snake and the solution exhibits a bright green emission color. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. PMC dosage is just 300600 mg at a time, 23 times per day. Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. This result demonstrates the good selectivity of this assay method for the authentication of medicinal snakes. MeSH and Nguyen, T.Q. Stability: A kit kept at -20C was randomly selected and Vaagt, F., Haase, I. Pharmacopoeia, the official recipe for the mixture is 1 part snake bile The powdered snake is All rights reserved. Because there are some snakes that are now endangered species, Would you like email updates of new search results? bp in the gel electrophoretogram of Zaocys dhumnades tested 2d. The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. period derived from the Indian culture. the treatment of superficial diseases, including skin eruptions, eye infections Hist. In fact, aside from Chinese doctors working in the U.S. and prescribing and Natrix trigrina (water specific DNA sequences, so that the method is especially enough [7]. Zhang YS, Cao QP, Zhang YS. Mater. . 1987. Chao, Z. et al. Would you like email updates of new search results? Environ. Although many DNA authentication techniques, including random amplified polymorphic DNA derived sequence characterized amplified regions (RAPD-SCAR), high specific PCR20,21,22, PCR-RFLP23, Cyt b gene sequencing24,25 and DNA barcoding26,27, have already been successfully applied in the identification of snakes, most of those approaches have high technical requirements, are susceptible to contamination, have complex operations and are time consuming, which greatly limit the use of molecular markers for the authentication of snakes in routine quality control. For the mean, error bars indicateS.D. How to cite this article: Jiang, C. et al. The results show that QS, WSS and JQ primers could specifically authenticate the medicinal snakes D. acutus, Z. dhumnades and B. multicinctus. Mag. Mater. The head in the center of the disc, flattened rounded. Keep away from direct sunlight and heat. thawing 20 times. Portland, Oregon. Two types of snakes, agkistrodon and zaocys, (6) 1: 165-172 -, Herrmann, H-J., Kabisch, K. & Khnel, K.-D. 1995. 5, 333336 (2007). An official website of the United States government. control did not show the amplified band, suggesting that kit Herpetology of Japan and adjacent territory. /*

South Today Presenters, External Factors Affecting Educational Achievement Sociology, Articles Z